DNA
BsaI ->
Part:BBa_K1362424:Experience
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-07)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1362424
- Team:iGEM-IISERPUNE-2020
- We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021.
User Reviews
UNIQe9aaea860536ddd8-partinfo-00000000-QINU UNIQe9aaea860536ddd8-partinfo-00000001-QINU